NMRNMR Spectra

About NMR

Sample Proton NMR Spectra

NMR (Nuclear Magnetic Resonance) or FT-NMR technique measures RF (radio frequency) absorption by the NMR active isotope of an element when placed in a high magnetic field. The isotope is ‘NMR Active’ when it possesses the property ‘SPIN’ (I) higher than zero (I > 0). In other words, the nuclear isotope must have spin angular momentum for it to have a magnetic moment and to give a NMR signal and to show a NMR spectrum.

For example, hydrogen has 3 NMR Active isotopes known as Proton (H-1, SPIN=1/2), Deuterium (D, H-2, SPIN=1) and Tritium (T, H-3, SPIN=1/2) which is radioactive. We can measure both Proton and Deuterium NMR from a sample of liquid water. The nuclear abundance of radioactive Tritium is so small that it requires isotopic enrichment with tritium in a sample of water to see Tritium NMR.

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NMR Spectra are better classified based on the NMR active nucleus (see our handy NMR Periodic Table) that is detected after tuning the detection coil in the probe for the maximum absorption of radio frequency (RF) radiation appropriate for that isotope in the magnetic field of the superconducting magnet of the NMR spectrometer.

Each NMR Active nuclear isotope absorbs RF radiation at different radio frequency with different intensity based mainly on its magnetic property and natural abundance unless the sample is isotopically enriched with the desired isotope. NMR spectra and its sensitivity or signal strength, therefore, varies with the nuclear isotope measured.

For example, it is not uncommon to measure Nitrogen NMR from the naturally abundant N-15 isotope of a compound, such as ‘Formamide’. However, in common practice, Nitrogen NMR of most Proteins, DNA and RNA samples are measured after enriching the samples with N-15 isotopes.

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There are many NMR active nuclei in molecules that give reasonable NMR spectra for analysis:

NMR Spectra Samples of Most Common Nuclei
PROTON NMR (H-1)	DEUTERIUM NMR (D, H-2)	CARBON NMR (C-13)
PHOSPHOROUS NMR (P-31)	FLUORINE NMR (F-19)	SILICON NMR (Si-29)

NMR Spectra Samples of Less Common Nuclei
LITHIUM NMR (Li-6, Li-7)	BERRELIUM NMR (Be-9)	BORON NMR (B-11)
NITROGEN NMR (N-15)	OXYGEN NMR (O-17)	SODIUM NMR (Na-23)
MAGNESIUM NMR (Mg-25)	ALUMINUM NMR (AL-27)	SULPHUR NMR (S-33)
CHLORINE NMR (Cl-35)	POTASSIUM NMR (K-39)	CALCIUM NMR (Ca-43)
SCANDIUM NMR (Sc-45)	TITANIUM NMR (Ti-47, Ti-49)	COBALT NMR (Co-59)
SELENIUM NMR (Se-77)	BROMINE NMR (Br-79)	RUBIDIUM NMR (Rb-87)
STRONTIUM NMR (Sr-87)	YITTRIUM NMR (Y-89)	MOLIBDENUM NMR (Mo-95)
RHODIUM NMR (Rh-103)	CADMIUM NMR (Cd-113)	TIN NMR (Sn-117, Sn-119)
ANTIMONY NMR (Sb-121)	TELLURIUM NMR (Te-125)	TUNGSTEN NMR (W-183)
PLATINUM NMR (Pt-195)	THALIUM NMR (Tl-205)	LEAD NMR (Pb-207)
BISMUTH NMR (Bi-209)	NMR ()	NMR ()

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Choose the NMR spectrum from the following list to see corresponding sample NMR spectra:

Most Common NMR Spectra Samples

Proton NMR (H-1) Spectra Examples

• 1D Proton NMR
• 2D Proton-Proton COSY NMR
• 2D Proton-Carbon HSQC NMR
• 2D Proton-Carbon HMBC NMR

1D Proton NMR of γ-Tocotrienol

2D COSY 1D NMR of γ-Tocotrienol

2D HSQC 1D NMR of γ-Tocotrienol

2D HMBC NMR of γ-Tocotrienol

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Deuterium NMR (D, H-2) Spectra Examples

• 1D Deuterium NMR

Deuterium NMR of Benzene

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Carbon NMR Spectra Examples

• 1D Carbon NMR
• 1D DEPT-135 NMR
• 1D DEPT-90 NMR

1D Carbon NMR of γ-Tocotrienol

1D Carbon DEPT-135 NMR of γ-Tocotrienol

1D Carbon DEPT-90 NMR of γ-Tocotrienol

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Phosphorous NMR Spectra Examples:

• 1D Phosphorous NMR

Phosphorous NMR of Lignin

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Fluorine (F-19) NMR Spectra Examples

• 1D Proton-decoupled Fluorine NMR
• 2D Fluorine-Fluorine Correlation NMR
• 2D Fluorine-Proton Correlation NMR
• 2D Fluorine-Carbon Correlation NMR

1D Fluorine-19 NMR of C6F5H

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Silicon (Si-29) NMR Spectra Examples

• 1D Silicon NMR

Silicon-29 NMR of TMS Standard

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ChemAnalytical NMR Periodic Table

Click on the Nuclear Isotope for more information about the nucleus, its NMR properties and NMR Spectra

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